Cloning and sequence analysis of a full-length cDNA of SmPPlcb encoding turbot protein phosphatase 1 beta catalytic subunit     

亓飞  郭华荣  王建 《中国海洋湖沼学报》2008,26(1):54-61

Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 （PP1） is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study, a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase l（PPlcb）, was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus, designated SmPPlcb, by the rapid amplification of cDNA ends （RACE） technique. The cDNA sequence of SmPPlcb we obtained contains a 984 bp open reading frame （ORF）, flanked by a complete 39 bp 5＇ untranslated region and 462 bp 3＇ untranslated region. The ORF encodes a putative 327 amino acid protein, and the N-terminal section of this protein is highly acidic, Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp, a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B （PP2B）. And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that, SmPPlcb is extremely conserved in both amino acid and nucleotide acid levels compared with the PPlcb of other vertebrates and invertebrates, and its Kozak motif contained in the 5＇UTR around ATG start codon is GXXAXXGXXATGG, which is different from mammalian in two positions A6 and G3, indicating the possibility of different initiation of translation in turbot, and also the 3＇UTR of SmPPlcb is highly diverse in the sequence similarity and length compared with other animals, especially zebraf＇lsh. The cloning and sequencing of SmPPlcb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot.  相似文献   

硒对华贵栉孔扇贝碱性磷酸酶的酶动力学及理化性质研究          下载免费PDF全文

余群  王重刚  陈荣  郁昂  郑微云 《海洋科学》2008,32(10):56-61

探讨了微量硒(Se~(4+))对华贵栉孔扇贝(Chlamys nobilis(Reeve))碱性磷酸酶(AKP)的作用。以催化动力学原理研究了不同缓冲系统中微量硒时AKP的影响。结果表明,Se~(4+)对谈酶的作用与酶所处的环境有关。在0.05 mol/L Na_2CO_3-NaHCO_3(pH 9.0)缓冲溶液中Se~(4+)浓度小于12.5 mmol/L时呈现出激活作用;Se~(4+)浓度大于12.5 mmol/L时呈现出非竞争性的抑制作用,其抑制常数为6.81×10~(-2)mol/L。在0.05 mol/L Tris-HCl缓冲系统(pH 9.0)中,微量Se~(4+)对AKP具有强烈竞争性抑制作用,其抑制常数为3.79×10~(-1)mol/L。经紫外吸收光谱、荧光发射光谱和圆二色谱的实验表明,微量Se~(4+)与AKP作用后,该酶的构象已发生了变化。研究发现海水环境中若含有微量Se~(4+)(SeO_3~(2-)),可以促进扇贝AKP的水解。利于贝类的生长发育和外壳的形成,并有益于贝类的繁殖。  相似文献   

中国两性生殖卤虫各品系碱性磷酸酶、酸性磷酸酶同工酶基因的表达特征     

侯林  王熠  邹向阳 《海洋学研究》2000,18(4):22-28

采用聚丙烯酰胺凝胶垂直板电泳法 ,对中国两性生殖卤虫 Artemia sinica的 1 4个品系的碱性磷酸酶、酸性磷酸酶同工酶基因的表达特征进行了分析 ,并与 Artemia urmiana的碱性磷酸酶、酸性磷酸酶同工酶基因的表达特征进行了比较研究。结果表明 :中国两性生殖卤虫各品系的 ALP、 ACP同工酶的酶谱相同 ,均为单体酶。 ALP、 ACP同工酶分别由 ALP - 1、 ACP- 1 ,ALP- 2、ACP- 2 ,ALP- 3、ACP- 3三个基因座位编码 ,ALP- 1、ACP- 1座位存在a、 b两个共显性等位基因 ,ALP- 2、ACP- 2存在 a、b、c三个等位基因 ,ALP- 3、ACP- 3座位存在 a、b两个等位基因。共发现 7种基因型 :1 .0 / cc/ bb,2 .0 / bb/ bb,3.0 / aa/ aa,4.0 /ab/ aa,5.0 / ac/ ab,6.0 / ac/ aa,7.ab/ ab/ aa。仅在 A.urmiana中发现 0 / cc/ bb、 0 / bb/ bb基因型 ,而 A.sinica的主要基因型为 :0 / ac/ aa、 0 / ab/ aa、 0 / aa/ aa。仅 GN品系的 ALP - 1、 ACP- 1有基因座位表达。  相似文献   

Emplacement of the La Peña alkaline igneous complex,Mendoza, Argentina (33° S): Implications for the early Miocene tectonic regime in the retroarc of the Andes     

《Journal of South American Earth Sciences》2014

The La Peña alkaline complex (LPC) of Miocene age (18–19 Ma) lies on the eastern front of the Precordillera (32°41ʹ34ʺS, 68°59ʹ48″W, 1400–2900 m a.s.l.), 30 km northwest of Mendoza city, Argentina. It is a subcircular massif of 19 km² and 5 km in diameter, intruded in the metasedimentary sequence of the Villavicencio Formation of Silurian-Devonian age. It is the result of integration of multiple pulses derived from one or more deep magma chambers, which form a suite of silicate rocks grouped into: a clinopyroxenite body, a central syenite facies with a large breccia zone at the contact with the clinopyroxenite, bodies of malignite, trachyte and syenite porphyry necks, and a system of radial and annular dikes of different compositions. Its subcircular geometry and dike system distribution are frequent features of intraplate plutons or plutons emplaced in post-orogenic settings. These morphostructural features characterize numerous alkaline complexes worldwide and denote the importance of magmatic pressures that cause doming with radial and annular fracturing, in a brittle country rock. However, in the LPC, the attitude of the internal fabric of plutonic and subvolcanic units and the preferential layout of dikes match the NW–SE extensional fractures widely distributed in the host rock. This feature indicates a strong tectonic control linked to the structure that facilitate space for emplacement, corresponding to the brittle shear zone parallel to the N–S stratigraphy of the country rock. Shearing produced a system of discontinuities, with a K fractal fracture pattern, given by the combination of Riedel (R), anti-Riedel (R′), (P) and extensional (T) fracture systems, responsible for the control of melt migration by the opening of various fracture branches, but particularly through the NW–SE (T) fractures. Five different pulses would have ascent, (1) an initial one from which cumulate clinopyroxenite was formed, (2) a phase of mafic composition represented by dikes cross-cutting the clinopyroxenite, (3) a malignite facies that causes a small breccia in the clinopyroxenite, (4) a central syenite facies that develops breccias at the contact with the clinopyroxenite and, finally, (5) porphyry necks and a system of radial dikes intruding all units. At the moment of the emplacement different mechanisms would have acted, they summarized in: 1) opening of discontinuities synchronous to the magma circulation as the principal mechanism for formation of dikes and conduits; 2) stoping processes, that play an important role in the development of the breccia zone and enabling an efficient transference of material during the emplacement of the syenitic magma and 3) shear-related deformation (regional stress), affected the internal fabric of the facies, causing intracrystalline deformation and submagmatic flow, which is very evident in the central syenite intrusive. The kinematic analysis of shear planes allows proposing that emplacement of the LPC took place in a transtensive regime, which would have occurred in the back-arc of the Andes orogen, during a long period spanning from Miocene to the present, of the compressive deformation responsible, westward and at the same latitude, for the development of the Aconcagua fold and thrust belt.  相似文献   

青藏高原东北缘柳坪新生代苦橄玄武岩地球化学及其大陆动力学意义   总被引：3，自引：2，他引：1  

赖绍聪  秦江锋  赵少伟  朱韧之 《岩石学报》2014,30(2):361-370

柳坪苦橄玄武岩出露在青藏高原东北缘特殊的构造部位,位于青藏、华北和扬子三大构造域的交接转换区域。岩石形成年龄在23~7.1Ma之间,属于新近纪火山岩。岩石SiO2介于41.72%~42.82%之间,Na2OK2O,K2O/Na2O平均0.51,为一套典型的幔源钠质碱性玄武岩类。岩石微量及稀土元素具板内火山岩特征,Th、Rb等元素呈较明显的富集状态,而岩石显著的低K2O特征(0.48%~0.90%)明显不同于青藏高原北缘新生代钾质-超钾质火山岩系列。岩石87Sr/86Sr(0.704158~0.704668)、143Nd/144Nd(0.512831~0.513352)、206Pb/204Pb(18.729871~18.779184)、207Pb/204Pb(15.591395~15.602454)和208Pb/204Pb(39.097372~39.181458)等同位素变化特征具有显著的混源属性,投影点位于EMI、EMII、BSE及PREMA等典型地幔储库的过渡部位,并可能存在HIUM地幔源的部分参与,明显不同于单一地幔源局部熔融形成的玄武岩的同位素组成特征。表明新生代期间青藏东缘西秦岭-松潘地区受青藏、扬子及华北三大构造体系域的控制,西秦岭-松潘构造结处于从下部地幔到上部陆壳物质的总体汇聚拼贴阶段,地幔具有显著的混合特征。柳坪苦橄玄武岩正是在这种特定的构造背景下,由于新生代青藏高原软流圈地幔物质向东的流动,诱发西秦岭-松潘构造结多源混合的地幔橄榄岩局部熔融而形成。  相似文献   

Geochronology and geochemistry of Cretaceous Nanshanping alkaline rocks from the Zijinshan district in Fujian Province,South China: Implications for crust–mantle interaction and lithospheric extension     

《Journal of Asian Earth Sciences》2014

In situ zircon U–Pb ages and Hf isotopic data, major and trace elements, and Sr–Nd–Pb isotopic compositions are reported for Nanshanping alkaline rocks from the Zijingshan district in southwestern Fujian Province (the Interior or Western Cathaysia Block) of South China. The Nanshanping alkaline rocks, which consist of porphyritic quartz monzonite, porphyritic syenite, and syenite, revealed a Late Cretaceous age of 100–93 Ma. All of the rocks show high SiO₂, K₂O + Na₂O, and LREE but low CaO, Fe₂O₃^T, MgO, and HFSE (Nb, Ta, P, and Ti) concentrations. These rocks also exhibit uniform initial ⁸⁷Sr/⁸⁶Sr ratios of 0.7078 to 0.7087 and ε_Nd(t) values of −4.1 to −7.2, thus falling within the compositional field of Cretaceous basalts and mafic dikes occurring in the Cathaysia Block. Additionally, these rocks display initial Pb isotopic compositions with a ²⁰⁶Pb/²⁰⁴Pb_i ratio of 18.25 to 18.45, a ²⁰⁷Pb/²⁰⁴Pb_i ratio of 15.63 to 15.67, and a ²⁰⁸Pb/²⁰⁴Pb_i ratio of 38.45 to 38.88. Combined with the zircon Hf isotopic compositions (ε_Hf(t) = −11.7 to −3.2), which are different from those of the basement rocks, we suggest that Nanshanping alkaline rocks were primarily derived from a subduction-related enriched mantle source. High Rb/Sr (0.29–0.65) and Zr/Hf (37.5–49.2) but relatively low Ba/Rb (4.4–8.1) ratios suggest that the parental magmas of these rocks were most likely formed via partial melting of a phlogopite-bearing mantle source with carbonate metasomatism. The relatively high SiO₂ (62.35–70.79 wt.%) and low Nb/Ta (10.0–15.3) ratios, positive correlation between SiO₂ and (⁸⁷Sr/⁸⁶Sr)_I, and negative correlation between SiO₂ and ε_Nd(t) of these rocks suggest that the crustal materials were also involved in formation of the Nanshanping alkaline rocks. Combined with geochemical and isotopic features, we infer magmatic processes similar to AFC (assimilation and fractional crystallization) involving early fractionation of clinopyroxene and olivine and subsequent fractionation of biotite-dominated assemblages coupled with a lesser amount of crustal contamination, thereby forming the Nanshanping alkaline rocks. The Nanshanping alkaline rocks appear to be associated with an extensional environment in the Cathaysia Block. This extensional regime could have resulted in the slab break-off and rollback of the subducting paleo-Pacific plate and the upwelling of the asthenospheric mantle, which induced partial melting of the enriched lithospheric mantle in the Cretaceous.  相似文献   

低盐胁迫对大黄鱼非特异性免疫酶活力的影响          下载免费PDF全文

何亮银  史晓丽  周逢芳  陈美霞  韩坤煌 《应用海洋学学报》2022,41(2):347-354

以大黄鱼（Larimichthys crocea）为研究对象,研究急性、慢性低盐度胁迫对大黄鱼存活状况及非特异性免疫酶活力的影响。结果表明：急性低盐（15、8）胁迫下,在7 d的实验周期中,大黄鱼肝脏的超氧化物歧化酶（superoxide dismutase, SOD）活力呈先上升后下降的趋势,在第1天显著上升（p<0.05）后逐渐下降至显著低于对照组水平;肝脏的过氧化氢酶（catalase, CAT）活力整体呈先下降后上升再下降的趋势,在第1天显著下降后开始升高,第3天后开始下降,至第7天时CAT活力仍显著低于对照组水平;血清中的酸性磷酸酶（acid phosphatase, ACP）活力呈下降后逐渐升高的趋势,在第1 天时显著下降后逐步升高,至第7天时仍显著低于对照组;而血清中的碱性磷酸酶（alkaline phosphatase, ALP）活力在实验开始的第1天到第3天逐渐升高,且均显著高于对照组,至第7天时开始下降至对照组水平;血清中的溶菌酶（lysozyme, LZM）含量呈波动变化,整体呈先上升后下降趋势。慢性低盐（8）养殖14 d后,大黄鱼的各项非特异性免疫酶活力均与对照组无显著性差异（p>0.05）。此外,实验周期内所有组别大黄鱼均未出现死亡,仅急性低盐胁迫组大黄鱼的活动和摄食受到有限影响。大黄鱼对慢性降盐度养殖有较高的耐受能力,而盐度骤降会显著影响大黄鱼的非特异性免疫,实际生产中应避免养殖环境盐度的剧烈变化。  相似文献   

A Discussion of Concavo-Convex Contacts between Oolites and Quartz Grains in Mixed Siliciclastic-Carbonate Rocks in Huanghua Basin, China     

Ma Yanping  Liu Li 《世界地质(英文版)》2002,5(2)

Concavo-convex contacrs between oolites and quartz grains are observed in mixed siliciclastic-carbonate rocks of the lower part of number 1 of Shahejie Formation, beach area, Dagang. Thin sections observation shows that oolite is convex in quartz grain, and oolite is complete. A possible explanation for it is pressure dissolution under alkaline fluid.  相似文献   

东营凹陷北部沙河街组四段深部储层多重成岩环境及演化模式   总被引：7，自引：0，他引：7       下载免费PDF全文

张善文  袁静  隋风贵  陈鑫 《地质科学》2008,43(3):576

主要通过薄片鉴定、扫描电镜观察、包裹体分析、粘土矿物分析、镜质体反射率测试和岩心物性分析等手段,结合构造发育史和有机质热演化史等研究成果,以揭示东营凹陷北带古近系深部碎屑岩储层成岩环境及演化模式为目的展开研究工作。结果表明:东营凹陷北带古近系深部碎屑岩储层存在酸性、碱性和酸性碱性交替等多重成岩环境。酸性成岩环境以碳酸盐矿物溶解、长石溶解蚀变为高岭石并伴生石英次生加大等为标志,碱性成岩环境以石英质颗粒及其次生加大边溶解、长石次生加大和晚期碳酸盐矿物沉淀为标志。研究区古近系深部碎屑岩储层成岩环境由浅至深大致经历了碱性—酸性—酸性碱性交替(局部碱性较强)—碱性—弱碱性的演化过程,并建立了沙河街组四段的成岩演化和储层改造模式。  相似文献   

Ecological significance of alkaline phosphatase activity and phosphatase-hydrolyzed phosphorus in the northern part of Gamak Bay, Korea   总被引：1，自引：0，他引：1  

Kwon HK  Oh SJ  Yang HS 《Marine pollution bulletin》2011,62(11):2476-2482

We investigated the ecological significance of alkaline phsophatase (APase) and alkaline phosphatase-hydrolyzable phosphorus (APHP) in the northern part of Gamak Bay, Korea. APase activity was detectable throughout the year, and dissolved inorganic phosphorus (DIP) concentration and APase activity are highly correlated and can be regarded as an indicator of DIP-limiting conditions. Also, a strong linear positive correlation between APase activity and Chl a concentration indicated that the major part of APase activity may have been induced by phytoplanktons. The APHP proportion in dissolved organic phosphorus (DOP) was above ca. 30% from winter to spring and below ca. 15% from summer to autumn due to freshwater discharge and uptake by phytoplankton. APHP may play an important role in species competition in coastal area such as northern part of Gamak Bay where DIP is limiting. Thus, APase induction by phytoplanktons may be ecologically significant, allowing dominance by these organisms under DIP-limiting conditions.  相似文献